Javascript er ikke aktivert i din nettleser. Dette er nødvendig for å bruke Oncolex. Kontakt din systemadministrator for å aktivere JavaScript.

Ultrasound-guided fine-needle aspiration and biopsy from the pelvis

Implementation

Ultrasound-guided biopsy from the pelvis is taken usually under general anesthesia, but fine-needle aspiration for cytology examination can be taken without anesthesia.

Cytology sample (fine-needle aspiration)

  • Wash the vagina with chlorhexidine 1 mg/ml
  • Localize the lesion with the ultrasound probe
  • Determine the best point of puncture and direction. Use a puncture line.
  • Puncture the spinal needle quickly through mucous membrane.
  • Insert the needle into the lesion using ultrasound.
  • Pull out the mandrin.
  • Insert the needle into the lesion.
  • Aspirate while the needle moves back and forth 2-3 times per second until the material is visible in the upper part of the needle.
  • Retract the suction and pull the needle out.
  • Deposite the specimen onto the slide

Spread for cytology

  • Spread the specimen onto the slide.
  • Dry the specimen under a fan or hairdryer.
  • Alternative 1: send the specimen unfixed to the cytologist.
  • Alternative 2: Immediate staining and assessment.
    • Fixation solution with methanol + haemacolor + rinse in water
    • 5 dips in fixer. Allow the solution to drip off onto paper.
    • 3 dips in stain solution 1.
    • 6 dips in stain solution 2. Allow the solution to drip onto paper.
    • Rinse in 2 baths of clean water.
  • Examine the specimen under the microscope with 10x or 20x objective.
  • Microscopic assessment of the cell material should be done by a cytologist to determine if supplementary samples are needed. 

Histological biopsy (pistol biopsy)

  • For pistol biopsy, general anesthesia is used.
  • Insert the biopsy needle up to the lesion and insert slightly, depending on the size and type of the tissue in front and behind the lesion.
  • The needle moves 2.5 cm forward when released.
  • Retract the pistol such that the sample is taken.
  • Retrieve the needle out and open it.
  • Place the piece of tissue on a slide with transfer medium, for example formalin.  

Oslo University Hospital shall not be liable for any loss whether direct, indirect, incidental or consequential, arising out of access to, use of, or reliance upon any of the content on this website. Oslo University Hospital© 2018