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Fine needle biopsy, non-aspiration technique


The patient should sit or lie on the examination table - whatever gives the best result. 

  • Wash the area for puncture with colorless chlorhexidine 1 mg/ml.
  • Allow the skin to dry.
  • Palpate the node/tumor.
  • Find the best position for puncturing.
  • Fix the lymph node/tumor between your fingers.
  • Puncture quickly through the skin with the cannula.
  • Move the cannula back and forth into the node in different directions (approximately 2–3 movements/second).
  • When (after 3–4 seconds) the material is visible in the upper part of the cannula passage, the cannula is retrieved.
  • Put a pressure on the point of puncture if needed.
  • Connect the cannula to the syringe filled with air.
  • Carefully spray the specimen from the cannula onto the slide.
  • If the suspicion of lymphoma or tumor is difficult to confirm, repuncture and put some material in the RPMI medium from a new puncture (for flow cytometry/molecular examination etc. Which type of examination is determined after microscopic examination.)
  • Smear the specimen on the slide.
  • Dry the specimen under a fan or hairdryer.
  • Staining: fixation fluid with methanol + haemacolour + rinsing in water  
    • 5 dips in fixation fluid. Allow the solution to drip off on paper. 
    • 3 dips in staining solution 1.
    • 6 dips in staining solution 2. Allow the solution to drip off onto paper.
    • Rinse in two tubs with clean water.
  • Examine the specimen under the microscope with a 10X or 20X objective.



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