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Immunophenotyping with flow cytometry

Implementation

  • A smear of the test material is made and stained for a microscopic examination.
  • The specimen is washed with physiological saline to remove plasma proteins which can interfere with antibody-antigen binding.  

From the microscopy assessment, clinical information and patient history, it is determined which combinations of antibodies will be used for staining in each case.

  • The red blood cells are hemolysed leaving a concentrate consisting of only white blood cells.
  • The specimen is analyzed by the flow cytometer.
  • The raw data are analyzed using dedicated software.

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