A. Kathrine Lie MD
Recently updated 18.09.2011
Oslo University Hospital
Preparing a prostate for pathologic analysis
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- The pathologist weighs, measures, describes the external appearance.
- A probe is placed through the urethra to better orient the prostate.
- The surface of the prostate is then dyed. The dorsal surface is dyed black, the left lobe blue, and right lobe green.
- The probe is then removed and the prostate is dried. The seminal vesicals are then cut from the prostate.
- A small end from the apax and base are then cut, and the apex and base are separated through the urethra.
- A sagittal section is made from each half in the apex and base. A cross section is then made of the middle part as a large section.
- A cross section from the seminal vesicals is then made.
- Calcificationsare removed if necessary before the sections are placed in blocks with the referral number and block number.
- All of the sections are fixed for an additional 48 hours in formalin before further processing.
- The blocks are then placed in a processing machine where the tissue is dehydrated, rinsed, and infiltrated with paraffin using alcohol, xylenes, and paraffin.
- The tissue is then embedded in liquid paraffin that solidifies into a block allowing to make very thin slices of tissue.
- The paraffin blocks are stored in a freezer until the sections are made.
- The technician slices 1-2 μmm sections which are transferred to a slide glass in a water bath.
- The sections are then transferred to a staining maching where the paraffin is removed and they are stained with haematoxylin and eosin, dehydrated, and mounted.
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